課程大綱

課程資訊

課程名稱	動物基因轉殖與複製技術 Transgenic and Cloning Technology in Animal
開課學期	103-2
授課對象	生物資源暨農學院生物科技研究所
授課教師	宋麗英
課號	Biot8005
課程識別碼	642ED0060
班次
學分	3
全/半年	半年
必/選修	選修
上課時間	星期三5,6,7(12:20~15:10)
上課地點
備註	本課程以英語授課。教室:R413. 限博士班總人數上限：7人
Ceiba 課程網頁	http://ceiba.ntu.edu.tw/1032Biot8005_spring2
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課程概述	COURSE OUTLINES AS FOLLOWING: PART I : TRANSGENIC ANIMALS 1. INTRODUCTION OF TRANSGENIC ANIMALS 2. GENERATION OF TRANSGENIC ANIMALS 3. FACTORS OF AFFECTING TRANSGENIC ANIMAL PRODUCTION 4. TRANSGENIC ANIMAL MODEL SYSTEM 5. APPLICATIONS OF TRANSGENIC FARM ANIMALS PART II: SOMATIC CELL NUCLEAR TRANSFER (SCNT) IN ANIMAL 6. AN OVERVIEW OF STRATEGIES TO INDUCE EPIGENETIC REPROGRAMMING 7. INTRODUCTION OF NUCLEAR REPROGRAMMING BY SOMATIC CELL NUCLEAR TRANSFER 8. SOMATIC NUCLEAR TRANSFER IN RODENT SPECIES 9. SOMATIC NUCLEAR TRANSFER IN DOMESTIC ANIMAL SPECIES 10. INTERSPECIES NUCLEAR TRANSFER (INT) AND ITS IMPLICATIONS FOR EMBRYONIC STEM CELL BIOLOGY 11. EPIGENETIC REGULATION IN CLONED EMBRYO DEVELOPMENT 12. DERIVATION AND CHARACTERIZATION OF ESCS FROM CLONED EMBRYOS 13. APPLICATIONS OF CLONING, REPRODUCTIVE VS. THERAPEUTIC CLONING 14. ETHICAL CONCERNS OF ANIMAL CLONING AND EMBRYONIC STEM RESEARCH
課程目標	ANIMAL TRANSGENESIS AND NUCLEAR TRANSFER (NT) TECHNOLOGY ARE POWERFUL TOOLS FOR UNDERSTANDING THE CELLULAR AND MOLECULAR ASPECTS OF GENE EXPRESSION IN EMBRYO DEVELOPMENT. THIS COURSE WILL PROVIDE STUDENTS A STATE OF THE ART OF ANIMAL TRANSGENESIS AND SOMATIC CELL NUCLEAR CLONING, AS WELL AS ITS APPLICATIONS. STUDENTS WILL BE ASKED TO PRESENT IN PARTICULAR TOPIC THEY WERE ASSIGN AFTER EXTENSIVE REVIEW AND DISCUSSION. GUEST SPEAKERS WILL PLAN TO INVITE FOR THE LECTURES AS NECESSARY.
課程要求
預期每週課後學習時數
Office Hours
指定閱讀
參考書目	1.IAN R. GORDON, REPRODUCTIVE TECHNOLOGIES IN FARM ANIMALS. 2004. CABI PUBLISHING, CABI INTERNATIONAL, WALLINGFORD, OXFORDSHIRE, UK 2.PAUL J. VERMA AND ALAN O. TROUNSON, NUCLEAR TRANSFER PROTOCOLS-CELL REPROGRAMMING AND TRANSGENESIS, HUMANA PRESS, TOTOWA, NEW JERSEY, USA
評量方式 (僅供參考)

課程進度

週次	日期	單元主題
Week 1	2/25	Course introduction and discussion
Week 2	3/04	Animal transgenesis – state of the art and applications
Week 3	3/11	Workshop- Equipments and reagents for mouse oocyte/embryo micromanipulations Discussion: Review for folliculogenesis, oogenesis, and embryogenesis
Week 4	3/18	Workshop - Bovine oocyte and embryo cryopreservation Discussion: Bovine oocyte and embryo cryopreservation– state of the art and applications
Week 5	3/25	Workshop - Mouse oocyte and embryo cryopreservation Discussion: Mouse oocyte and embryo cryopreservation– state of the art and applications
Week 6	4/01	Spring break
Week 7	4/08	Workshop - Mouse intracytoplasmic sperm injection (ICSI) Discussion: History of ICSI and its applications
Week 8	4/15	Workshop- Tetraploid embryo complementation and blastocyst injection Discussion: Pluripotent of stem cells
Week 9	4/22	Mid-exam Student presentation-
Week 10	4/29	Workshop- Derivation of mouse ES cells Discussion: Therapeutic cloning and its applications
Week 11	5/06	Workshop- Pronuclear DNA microinjection Discussion: Transgenic animals – state of the art and applications
Week 12	5/13	Workshop – Enucleation (squeeze) of bovine/rabbit oocyte / troubleshooting Discussion: SCNT and nuclear reprogramming in bovine & rabbit
Week 13	5/20	Workshop - Enucleation (aspiration) of mouse oocyte / troubleshooting Discussion: SCNT and nuclear reprogramming in mouse
Week 14	5/27	Workshop- Donor cell transfer –injection method/ troubleshooting
Week 15	6/03	Workshop- Donor cell transfer –fusion method/ troubleshooting
Week 16	6/10	Student presentation and discussion
Week 17	6/17	Student presentation and discussion
Week 18	6/24	Final exam: final report